Nc. Mao et al., The murine Bin1 gene functions early in myogenesis and defines a new region of synteny between mouse chromosome 18 and human chromosome 2, GENOMICS, 56(1), 1999, pp. 51-58
We cloned and functionally characterized the murine Bin1 gene as a first st
ep to investigate its physiological roles in differentiation, apoptosis, an
d tumorigenesis. The exon-intron organization of the greater than or equal
to 55-kb gene is similar to that of the human gene. Consistent with a role
for Bin1 in apoptosis, the promoter included a functional consensus motif f
or activation by NF-kappa B, an important regulator of cell death. A muscle
regulatory module defined in the human promoter that includes a consensus
recognition site for myoD family proteins was not conserved in the mouse pr
omoter. However, Bin1 is upregulated in embryonic development by E10.5 in m
yotomes, the progenitors of skeletal muscle, supporting a role in myogenesi
s and suggesting that the mouse and human genes may be controlled somewhat
differently during development. In C2C12 myoblasts antisense Bin1 prevents
induction of the cell cycle kinase inhibitor p21WAF1, suggesting that it ac
ts at an early time during the muscle differentiation program. Interspecifi
c mouse backcross mapping located the Bin1 locus between Mep1b and Ape on c
hromosome 18. Since the human gene was mapped previously to chromosome 2q14
, the location of Bin1 defines a previously unrecognized region of synteny
between human chromosome 2 and mouse chromosome 18. (C) 1999 Academic Press
.