MAGIC-ANGLE-SPINNING PROTON NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC ANALYSIS OF INTACT KIDNEY TISSUE SAMPLES

High resolution H-1 nuclear magnetic resonance (NMR) spectra using spi nning at the magic angle (H-1 MAS NMR) have been obtained on approxima tely 80 mg samples of intact human kidney tissue obtained during surge ry, This technique produces high resolution H-1 NMR spectra in which e ffects of dipolar couplings, chemical shift anisotropy and magnetic su sceptibility differences are minimised, giving resolution comparable t o solution state NMR spectroscopy, The combined use of one-dimensional single pulse and spin-echo spectra, and two-dimensional J-resolved an d total correlation (TOCSY) spectra, allowed the assignment of a numbe r of resonances of endogenous substances from both cytosolic and membr ane compartments, These are the first reported results on human tissue s using this technique and the approach offers potential for the ident ification and rapid quantitation of tissue components and determinatio n of changes in disease states, thereby providing scope for diagnosis and therapy evaluation.