Does glucose affect fertilization, development and pregnancy rates of human in-vitro fertilized oocytes?

The study was conducted to examine whether the presence of glucose in the i ncubation medium affects fertilization, development and implantation rates of human oocytes of patients who were attending our in-vitro fertilization programme. Harvested oocytes were transferred into one of four different me dia: human tubal fluid (HTF), P1, M3 and IVF-Universal (IVF-Med). Three of these contained glucose; the fourth (P1), contained no glucose or phosphate ions. In an independent preliminary study, some of the oocytes of each pat ient were incubated in IVF-Med, which lacks phosphate ions, but not glucose . Comparisons of fertilization rates between media pairs showed differences among all pairs except HTF and M3. When comparing the four study groups, n o difference was noticed in embryo development or embryo quality 48 h post- ovum retrieval. A higher development rate was demonstrated in embryos incub ated in M3 medium, in comparison with the pi and IVF-Med embryos after incu bation for 72 h. No difference in pregnancy rate was found after embryo tra nsfers of preimplantation embryos which were incubated in one of the follow ing media: HTF, M3 and IVF-Med (seven out of 22, 18 of 54 and 32 of 69 trea tment cycles respectively). A lower incidence of pregnancies occurred follo wing transfers of embryos which were incubated in pi medium (seven pregnanc ies out of 37 cycles). We suggest that the presence of glucose in the incub ation medium enhances implantation potential of in-vitro-developing preimpl antation embryos.