Comparison of catalytic activity of immobilized amyloglucosidase on DEAE-cellulose with native enzyme on dextrin-hydrolysis

Amyloglucosidase is an exo-enzyme which is used in the saccharification of starch in industries. In this study the catalytic activity of amyloglucosid ase immobilized on DEAE-cellulose (diethyl amino ethylcellulose) was invest igated and compared with native enzyme on hydrolysis of dextrin. Amylogluco sidase was immobilized on DEAE-cellulose by ionic binding method. Activity of DEAE-cellulose immobilized and native enzymes were compared and it was o bserved that immobilized amyloglucosidase has 78% of the catalytic activity of native enzyme. The activity of DEAE-cellulose immobilized enzyme as a f unction of pH and temperature was determined. Kinetic constants K-m and V-m ax were also determined.