As. Rani et al., Comparison of catalytic activity of immobilized amyloglucosidase on DEAE-cellulose with native enzyme on dextrin-hydrolysis, I J CHEM T, 5(6), 1998, pp. 397-401
Amyloglucosidase is an exo-enzyme which is used in the saccharification of
starch in industries. In this study the catalytic activity of amyloglucosid
ase immobilized on DEAE-cellulose (diethyl amino ethylcellulose) was invest
igated and compared with native enzyme on hydrolysis of dextrin. Amylogluco
sidase was immobilized on DEAE-cellulose by ionic binding method. Activity
of DEAE-cellulose immobilized and native enzymes were compared and it was o
bserved that immobilized amyloglucosidase has 78% of the catalytic activity
of native enzyme. The activity of DEAE-cellulose immobilized enzyme as a f
unction of pH and temperature was determined. Kinetic constants K-m and V-m
ax were also determined.