DNA probes specific for Aeromonas hydrophila (HG1)

Citation
Hj. Oakey et al., DNA probes specific for Aeromonas hydrophila (HG1), J APPL MICR, 86(2), 1999, pp. 187-193
Citations number
23
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN journal
13645072 → ACNP
Volume
86
Issue
2
Year of publication
1999
Pages
187 - 193
Database
ISI
SICI code
1364-5072(199902)86:2<187:DPSFAH>2.0.ZU;2-D
Abstract
Aeromonas hydrophila (HG1)-specific RAPD-PCR fragments were investigated fo r their potential as DNA probes. From 20 RAPD-PCR fragment bands, it was fo und that two were specific to all isolates of Aeromonas hydrophila (HG1) te sted. Cloning and nucleotide sequence determination of one of these bands s howed that co-migration of similar sized amplicons had occurred and that th is band (designated '7e') contained at least four fragments of different se quences. Three of these individual amplicons had a sequence specific to Aer . hydrophila (HG1) isolates. The sequence of one of these amplicons ('7e5') was used to design primers for a specific polymerase chain reaction (PCR). The specificity of the PCR was achieved using a modified hot-start procedu re. The identity of the PCR amplicons was confirmed by high stringency hybr idization with a digoxygenin-labelled 7e5 probe.