Retrieval, maturation, and fertilization of immature oocytes obtained fromunstimulated patients with polycystic ovary syndrome

Purpose: Our purpose was to determine whether immature oocytes could be ret rieved under local anesthesia, whether these oocytes would mature and ferti lize in vitro, and whether adequate endometrium development could be obtain ed after hormonal supplementation. Methods: Ovum pick-up was performed under local anesthesia. Immature oocyte s were cultured and inseminated. To prepare the endometrium, estradiolvaler ate was administered in combination with micronized progesterone. Results: Immature oocytes were obtained in all cases. Fifty-six percent (n = 30) of the oocytes developed into metaphase II (MII) after 48 hr of cultu re, and another 20% reached the MII stage by 72 hr: Normal fertilization wa s observed in only 10% of oocytes inseminated. No embryonic development occ urred and therefore embryo transfer was nor performed in any of the patient s. Endometrial microbiopsy was performed in all subjects and endometrial de velopment was considered sufficient in eight patients. Conclusions: We collected immature oocytes from patients with polycystic ov ary syndrome without general anesthesia. In vitro maturation of these oocyt es seemed adequate bur fertilization rates were poor Sufficient endometrial quality was obtained after hormonal substitution.