Influence of chondroitin sulfate charge density, sulfate group position, and molecular mass on Cu2+-mediated oxidation of human low-density lipoproteins: Effect of normal human plasma-derived chondroitin sulfate

The effects of chondroitin sulfate samples with decreasing charge densities , different 4-sulfate/6-sulfate ratios, and various molecular masses on Cu2 +-induced oxidation of human low-density lipoprotein (LDL) were evaluated b y monitoring conjugated diene formation and the tryptophan fluorescence kin etics. Low-sulfated chondroitin sulfate (CS) from beef trachea had a very s trong protective antioxidant effect. Quite similar behavior was observed fo r CS from pig trachea, and a fructose-containing polysaccharide with a chon droitin backbone from Escherichia coli was also strongly protective as to L DL oxidation, CS samples with decreasing charge densities proved effective in inhibiting LDL oxidation, A totally desulfated sample still exhibited a great capacity to protect LDL against oxidation, CS-4-sulfate samples (sulf ate to carboxyl ratio of 0.62, about 65% 4-sulfate groups and 5% g-sulfate groups) retained great ability to inhibit the Cu2+-mediated human LDL oxida tion, CS fractions with different molecular masses were examined as possibl e inhibitors of LDL oxidation, Samples with molecular masses lower than abo ut 8,570 (13-15 disaccharide units) were unable to protect human LDL from C u2+-induced oxidation, Similar results were obtained on studying the degrad ation of tryptophan residues of the LDL protein moiety resulting from Cu2complexation through amino acid residues. A low-sulfated CS (sulfate to car boxyl ratio of 0.41, a molecular mass of about 15,600) having effective ant i-oxidant properties as to metal-induced LDL oxidation was isolated from no rmal human plasma. The protective capacity as to Cu2+-mediated LDL oxidatio n of CS is discussed in relation to its structure, also considering the phy siological role of plasma CS in relation to factors that can alter its prop erties.