I kappa B-alpha enhances transactivation by the HOXB7 homeodomain-containing protein

Citation
A. Chariot et al., I kappa B-alpha enhances transactivation by the HOXB7 homeodomain-containing protein, J BIOL CHEM, 274(9), 1999, pp. 5318-5325
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
274
Issue
9
Year of publication
1999
Pages
5318 - 5325
Database
ISI
SICI code
0021-9258(19990226)274:9<5318:IKBETB>2.0.ZU;2-A
Abstract
Combinatorial interactions between distinct transcription factors generate specificity in the controlled expression of target genes. In this report, w e demonstrated that the HOXB7 homeodomain-containing protein, which plays a key role in development and differentiation, physically interacted in vitr o with I kappa B-alpha, an inhibitor of NF-kappa B activity. This interacti on was mediated by the I kappa B-alpha ankyrin repeats and C-terminal domai n as well as by the HOXB7 N-terminal domain. In transient transfection expe riments, I kappa B-alpha markedly increased HOXB7-dependent transcription f rom a reporter plasmid containing a homeodomain consensus-binding sequence. This report therefore showed a novel function for I kappa B-alpha, namely a positive regulation of transcriptional activation by homeodomain-containi ng proteins.