Identification of low density lipoprotein receptor-related protein-2/megalin as an endocytic receptor for seminal vesicle secretory protein II

The low density lipoprotein receptor-related protein-2/megalin (LRP-2) is a n endocytic receptor that is expressed on the apical surfaces of epithelial cells lining specific regions of the male and female reproductive tracts. In the present study, immunohistochemical staining revealed that LRP-2 is a lso expressed by epithelial cells lining the ductal region and the ampulla of the rat seminal vesicle. To identify LRP-2 ligands in the seminal vesicl e, we probed seminal vesicle fluid with I-125-labeled LRP-2 in a gel-blot o verlay assay. A 100-kDa protein (under non-reducing conditions) was found t o bind the radiolabeled receptor. The protein was isolated and subjected to protease digestion, and the proteolytic fragments were subjected to mass s pectroscopic sequence analysis. As a result, the 100-kDa protein was identi fied as the seminal vesicle secretory protein II (SVS-II), a major constitu ent of the seminal coagulum. Using purified preparations of SVS-II and LRP- 2, solid-phase binding assays were used to show that the SVS-II bound to th e receptor with high affinity (K-d = 5.6 nM). The binding of SVS-II to LRP- 2 was inhibited using a known antagonist of LRP-2 function, the 39-kDa rece ptor-associated protein RAP. Using a series of recombinant subfragments of SVS-II, the LRP-2 binding site was mapped to a stretch of repeated 13-resid ue modules located in the central portion of the SVS-II polypeptide. To eva luate the ability of LRP-2 to mediate I-125-SVS-II endocytosis and lysosoma l degradation, ligand clearance assays were performed using differentiated mouse F9 cells, which express high levels of LRP-2, Radiolabeled SVS-II was internalized and degraded by the cells, and both processes were inhibited by antibodies to LRP-2 or by RAP. The results indicate that LRP-2 binds SVS -II and can mediate its endocytosis leading to lysosomal degradation.