Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization

Authors
Chiarparin, E Pelupessy, P Cutting, B Eykyn, TR Bodenhausen, G
Citation
E. Chiarparin et al., Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization, J BIOM NMR, 13(1), 1999, pp. 61-65
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOMOLECULAR NMR
ISSN journal
09252738 → ACNP
Volume
13
Issue
1
Year of publication
1999
Pages
61 - 65
Database
ISI
SICI code
0925-2738(199901)13:1<61:NONMII>2.0.ZU;2-Q
Abstract
A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross-polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of N-15 labeled human ubiquitin. Inter- and intra-residue NOEs are c learly observed in a very time-effective manner. The signal intensities can be easily normalized.