E. George et al., Influence of growth medium composition on the lipolytic enzyme activity ofOphiostoma piliferum (Cartapip (TM)), J CHEM TECH, 74(2), 1999, pp. 137-140
Production of esterases (carboxyl esterase EC 3.1.1.1) and lipases (glycero
l ester hydrolase EC 3.1.1.3) by Ophiostoma piliferum (Cartapip(TM)), a fun
gus commercialized to decrease fatty acyl esters in wood, is described. The
influence of various combinations of carbon and nitrogen sources, in the g
rowth medium, was examined. Medium containing yeast extract as a nitrogen s
ource and olive oil as a carbon source was found to be optimal for extracel
lular esterase (221 U dm(-3)) and lipase (152 U dm(-3)) activities. Further
increases in those enzyme activities were achieved by decreasing medium pH
from 6.5 to 5.5 (esterase 508 U dm(-3); lipase 415 U dm(-3)) and increasin
g medium calcium content from 8 m mol dm(-3) to 160 m mol dm(-3) (esterase
4084 U dm(-3); lipase 508 U dm(-3)) (C) 1999 Society of Chemical Industry.