Rapid detection of a casomorphin and new casomorphin-like peptide from a peptic casein hydrolysate by spectral comparison and second order derivativespectroscopy during HPLC analysis

Detection of a casomorphin and a new casomorphin-like peptide from a casein enzymatic hydrolysate was achieved using second-order derivative spectra a nd UV-spectra comparison. In this method, synthetic casomorphins were used as standards. The beta 1-3 casomorphin and alpha 90-94 casomorphin-like, we re detected and rapidly purified with RP-HPLC coupled with photodiode array detector. This technique simplifies the identification and the purificatio n of bioactive peptides containing aromatic amino acids from complex hydrol ysates. It could be applied to follow up the evolution of these peptides du ring casein hydrolysis.