Hydroxyapatite sol was prepared by dispersing unheated hydroxyapatite micro
crystals into distilled water. A suspension of hydroxyapatite heated at 800
degrees C (HAp-800 degrees C) in distilled water was used as a comparative
material. These were characterized by X-ray diffractometry, SEM and electr
oconductivity measurements. 0.001 ml of each material was injected into the
upper first molar pulp of rats. After 7-14 days post-operatively, the grou
p with hydroxyapatite sol showed slight inflammation in the pulp cavities a
nd a highly calcified tubular dentine bridge was observed. Osteodentine had
developed and adhered to the surface of the hydroxyapatite microcrystals.
Matrix vesicles were observed in the extracellular matrix between collagen
fibrils and electron-dense granules. After 28 days, the inflammation comple
tely disappeared and tubular dentine was observed below the osteodentine. A
cid phosphates (ACPase) activity was detected along all biosynthesizing pat
hways in odontoblast-like cells. Localized dehydrogenate (LDH) activity ind
icated phagocytosis of hydroxyapatite microcrystals by odontoblasts. In the
group with HAp-800 degrees C after 7-14 days post-operatively, the inflamm
ation was moderate. After 28 days, relatively fewer calcified dentine tubul
es were observed. ACPase activity was detected only along the membranes bou
nding cytoplasmatic vacuoles. It was concluded that hydroxyapatite sol is a
safe biomaterial for use as dental pulp and induces early formation of den
tine-bridge more so than HAp-800 degrees C. (C) 1998 Kluwer Academic Publis
hers.