The dynamic organization of a mixed Langmuir film of glucose oxidase a
nd behenic acid has been studied. Samples of the mixed film transferre
d after different relaxation times were analyzed by UV-Vis and IR spec
troscopies and by lateral force microscopy. The results demonstrate th
at the enzyme molecules present at the air/water interface are progres
sively covered by the behenic acid film. Moreover, we observe an incre
ase of the quantity of glucose oxidase in the mixed film with time, sh
owing that the adsorption of glucose oxidase under the behenic acid fi
lm continues during and after the relaxation process. (C) 1997 Elsevie
r Science S.A.