ELECTROCHROMATOGRAPHIC SEPARATION OF PROTEINS

We have developed a modified electrochromatography system which minimi zes Joule heating at electric field strengths up to 125 V/cm. A non-li near equilibrium model is described which incorporates electrophoretic mobility, hydrodynamic flow velocity, and an electrically induced con centration polarization at the surface of the stationary phase. This m odel is able to provide useful estimates of protein retention time and velocity in a column packed with Sephadex gel and subjected to an ele ctric field. A correlation of electrophoretic mobility of peptide and proteins with respect to their charge, molecular mass, and asymmetry e nables the selection of solute target molecules for electrochromatogra phic separations. Good separation of protein mixtures have been obtain ed.