Prejunctional control of pH 6-induced bronchoconstriction by NK1, NK2, mu-opioid, alpha(2)-adrenoceptor and glucocorticoid receptors in guinea-pig isolated perfused lung
S. Auberson et al., Prejunctional control of pH 6-induced bronchoconstriction by NK1, NK2, mu-opioid, alpha(2)-adrenoceptor and glucocorticoid receptors in guinea-pig isolated perfused lung, J PHARM PHA, 50(8), 1998, pp. 899-905
This study investigated the release of calcitonin-gene related peptide-like
(CGRP) immunoreactivity and bronchoconstriction induced by pH 6 buffer in
guinea-pig isolated perfused lung.
Both pH 6-induced CGRP-like immunoreactivity and bronchoconstriction were c
ompletely abolished after systemic pretreatment with capsaicin. Pretreatmen
t with the NK2 receptor antagonist SR 48968 (5 x 10(-7) M) completely inhib
ited bronchoconstriction and significantly reduced the immunoreactivity ind
uced by the pH 6 buffer. The NK1 antagonist SR 140333 (5 x 10(-7) M) and, t
o a lesser extent the NK1 antagonist CP 96345, morphine (5 x 10(-6) M), the
alpha(2)-adrenoceptor agonist UK 14304 (10(-7) M) and betamethasone (10(-6
) M) significantly reduced both pH 6-induced bronchial response and CGRP-li
ke immunoreactivity overflow. The effects of morphine and UK14304 were part
ially reversed by naloxone (5 x 10(-5) M) and idazoxan (5 x 10(-5) M).
Therefore, NK1, NK2, mu-opioid, alpha(2)-adrenoceptor and glucocorticoid re
ceptors seemed to have a prejunctional action on pH 6 buffer-induced CGRP-l
ike immunoreactivity and bronchoconstriction.