Blue native page as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits

Citation
A. Nicke et al., Blue native page as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits, J RECEPT SI, 19(1-4), 1999, pp. 493-507
Citations number
21
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH
ISSN journal
10799893 → ACNP
Volume
19
Issue
1-4
Year of publication
1999
Pages
493 - 507
Database
ISI
SICI code
1079-9893(199901/07)19:1-4<493:BNPAAU>2.0.ZU;2-1
Abstract
Oligomerization of complete and incomplete combinations of rat muscle-type nicotinic acetylcholine receptor (nAChR) subunits in Xenopus oocytes was st udied by blue native PAGE and compared with acetylcholine-activated current in these cells. The rank order of expression level judged by current was a lpha(1)beta(1)gamma delta >> alpha(1)beta(1)gamma > alpha(1)beta(1)delta > alpha(1)gamma delta >>alpha(1)delta >>alpha(1)gamma. alpha(1) and alpha(1)b eta(1) were not functional. Protein complexes incorporating a heptahistidyl -tagged alpha(1) subunit were chromatographically purified from digitonin e xtracts of oocytes and resolved by blue native PAGE. In the absence of any co-expressed nAChR subunit, the majority of alpha(1) formed aggregates. Co- expression of beta(1) had no effect on alpha(1) aggregation, whereas both g amma and delta diminished alpha(1) aggregation in favor of discrete oligome rs: alpha(1) formed tetramers together with gamma and dimers, trimers, and tetramers together with delta. When alpha(1)gamma was complemented with bet a(1) to form a functional alpha(1)beta(1)gamma receptor, a small amount of a pentamer was found besides a prominent alpha(1)-His(7)beta(1)gamma trimer . Expression of the functional alpha(1)beta(1)delta receptor yielded marked amounts of a pentamer besides dimers and trimers. These results are discus sed in terms of the assembly model of Green and Claudio (Cell 74, 57-69, 19 94), substantiating that blue native PAGE is suited for the investigation o f ion channel assembly.