Background. Cigarette smoking accelerates atherosclerosis and restenosis af
ter vascular reconstruction. The mechanisms by which smoking alters vessel
structure after injury are unclear. This study examined the effects of ciga
rette smoking on endothelial regeneration, an important component of arteri
al remodeling.
Materials and methods. Adult male rats were subjected to balloon injury of
the thoracic aorta and exposed to mainstream cigarette smoke via a Griffith
-type smoking machine for 2 weeks. Control groups included rats which were
restrained in the machine but not smoked and a group not utilizing the mach
ine. Aortic reendothelialization was determined using Evan's blue staining
of the arterial surface. Serum levels of nitric oxide were measured to dete
rmine if smoke exposure altered this potential endothelial cell mitogen.
Results. Cigarette smoking increased aortic endothelial regeneration (78.4
+/- 4.6% vs 59.2 +/- 2.1%, P < 0.05) and was associated with an increase in
serum nitric oxide level (59.9 +/- 7.1 mu M vs 28.5 +/- 1.8 mu M, P < 0.05
). Daily restraint alone in the smoking machine had no effect on endothelia
l regeneration.
Conclusions. This is the first report on the effects of smoking on endothel
ial regeneration and demonstrates that smoking increases reendothelializati
on after large vessel injury and serum levels of nitric oxide, an EC mitoge
n. (C) 1999 Academic Press.