Mouse-to-rat testicle transplantation

This report details mouse-to-rat testicular transplantation with immediate revascularization. Donor preparation involved grafting a long segment of ao rta and inferior vena cava (IVC) containing the testicular artery and vein. The graft aorta and IVC were anastomosed to the rat aorta and IVC, respect ively. Vasovasostomy was completed and the scrotal epitheliums were anastom osed to draw the graft toward the host scrotal sac. Twenty-nine of 53 trans plants were determined to be viable. Histologically, 6- to 18-hr-old grafts displayed moderate to minimal polymorphonuclear neutrophil (PMN) infiltrat es. Ischemia set in somewhere between 18-24 hr postoperatively. Beyond 24 h r the grafts displayed progressive infiltration of PMN and perivascular and intertubular lymphocytes, disorganization of the germinal epithelium, and cessation of spermatogenesis, (C)1999 Wiley-Liss, Inc.