Tbh. Reusch et al., Microsatellite loci in eelgrass Zostera marina reveal marked polymorphism within and among populations, MOL ECOL, 8(2), 1999, pp. 317-321
Using an enriched genomic library, we developed seven (CT)(n)/(GA)(n) micro
satellite loci for eelgrass Zostera marina L. Enrichment is described and h
ighly recommended for genomes in which microsatellites are rare, such as in
many plants. A test for polymorphism was performed on individuals from thr
ee geographically separated populations (N = 15/population) and revealed co
nsiderable genetic variation. The number of alleles per locus varied betwee
n five and 11 and the observed heterozygosities for single loci ranged from
0.16 to 0.81 within populations. Mean allele lengths were markedly differe
nt among populations, indicating that the identified loci will be useful in
studying population structure in Z. marina. As the frequency of the most a
bundant multilocus genotype within populations was always < 1%, these loci
have sufficient resolving power to address clone size in predominantly vege
tatively reproducing populations.