Quorum sensing in Vibrio fischeri: elements of the luxl promoter

Although cell density-dependent regulation of the luminescence genes in Vib rio fischeri is a model for quorum sensing in Gram-negative bacteria, relat ively little is known about the promoter of the luminescence operon. The lu minescence operon is activated by the LuxR protein, which requires a diffus ible acyl-homoserine lactone signal, The lux box, a 20 bp inverted repeat, is located in the luxI promoter region and is required for LuxR-dependent i nduction of the luminescence genes, Using primer extension, we mapped the L uxR-dependent transcriptional start site of the lux operon to 19 bp upstrea m of the luxI start codon. This indicates that the lux box is centred at -4 2.5 bp from the start of transcription, To gain evidence about the location of the -10 sequence, we placed a consensus -35 hexamer at different locati ons relative to the luxI transcriptional start site and measured constituti ve levels of luminescence in recombinant Escherichia coli, The strongest co nstitutive promoter contained a TATAGT hexamer 17 bp from the -35 consensus sequence and 6 bp from the transcriptional start site, We propose that thi s is the -10 hexamer, Also in recombinant E. coil, both half-sites of the l ux box were required for LuxR-dependent gene activation and for activation by an autoinducer-independent, monomeric LuxR deletion protein, LuxR-depend ent activation of luminescence was eliminated when the lux box was centred at -47.5, -52.5 and -62.5 with respect to the luxI transcriptional start si te. Our evidence, taken together with other information, points to a model in which a LuxR dimer overlaps the -35 region of the luxI promoter and func tions as an ambidextrous activator with each LuxR subunit interacting with a different region of RNA polymerase.