Internalization and resistance to degradation of Alzheimer's A beta 1-42 at nanomolar concentrations in THP-1 human monocytic cell line

Citation
L. Morelli et al., Internalization and resistance to degradation of Alzheimer's A beta 1-42 at nanomolar concentrations in THP-1 human monocytic cell line, NEUROSCI L, 262(1), 1999, pp. 5-8
Citations number
22
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
03043940 → ACNP
Volume
262
Issue
1
Year of publication
1999
Pages
5 - 8
Database
ISI
SICI code
0304-3940(19990226)262:1<5:IARTDO>2.0.ZU;2-M
Abstract
Microglial cell involvement in Alzheimer's disease has been related to amyl oid beta (A beta) internalization, the release of inflammatory cytokines an d the development of neuritic plaques. The human monocyte/macrophage THP-1 cell line has been widely used as a model of human microglial cells. We use d THP-I cells to study the adsorption, internalization and resistance to de gradation of A beta 1-40 and A beta 1-42 isoforms offered at nanomolar conc entrations and free of large aggregates, conditions that may mimic a pre-fi brillar stage of A beta in the brain. Under these conditions, A beta s did not induce THP-1 activation, as assessed by interleukin-1 beta expression. A beta 1-42 showed a preferential adsorption and intracellular accumulation as compared to A beta 1-40, supporting that competent nuclei for A beta 1 -42 ordered aggregation may be formed inside microglial cells. In light of the possible neurotoxicity of soluble A beta 1 -42, we propose that amyloid formation within brain phagocytic cells may be a protective mechanism in e arly stages of the disease. (C) 1999 Published by Elsevier Science Ireland Ltd. All rights reserved.