Me. Palmer et al., Exact mass determination of narrow electrophoretic peaks using an orthogonal acceleration time-of-flight mass spectrometer, RAP C MASS, 13(4), 1999, pp. 256-263
High efficiency electrically driven separation techniques, such as capillar
y zone electrophoresis (CZE) and capillary electrochromatography (CEC), yie
ld narrow peak widths of the order of a few seconds. Scanning instruments m
ay have difficulty in obtaining sufficient data points across such narrow p
eaks (although the use of selected ion monitoring (SIM) mode may overcome t
his). Orthogonal acceleration time-of-flight ((oa-TOF)) mass analysers have
an inherent ability for fast acquisition rates allowing a high number of d
ata points (for example 10 spectra s(-1)) to be collected across a narrow p
eak and are capable of operation at resolutions in the region of 5000 full
width half maxims (FWHM), This enables spectral data to be measured with ac
curacies greater than 5 ppm, allowing the molecular formula to be predicted
/ confirmed. We have investigated the use of an oa-TOF mass analyser coupl
ed to CZE, The on-line separation of a drug substance and related impuritie
s has been achieved. Copyright (C) 1999 John Wiley & Sons, Ltd.