CMS sources in sunflower: different origin but same mechanism?

Authors
Citation
R. Horn et W. Friedt, CMS sources in sunflower: different origin but same mechanism?, THEOR A GEN, 98(2), 1999, pp. 195-201
Citations number
22
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
THEORETICAL AND APPLIED GENETICS
ISSN journal
00405752 → ACNP
Volume
98
Issue
2
Year of publication
1999
Pages
195 - 201
Database
ISI
SICI code
0040-5752(199902)98:2<195:CSISDO>2.0.ZU;2-6
Abstract
The presence of orfH522, orfH708 and orfH873 in the mtDNA, as well as the e xpression of mitochondrially encoded proteins, were investigated for 2X sou rces of cytoplasmic male sterility (CMS) and HA89, a fertile line of Helian thus annuus. The whole 5-kb insertion, found in PET1, is also present in al l PET1-like CMS sources. However, with regard to the 11-kb inversion ANO1 d emonstrated a different organization at the cob locus from the other PET1-l ike CMS sources. Only orfH873 gave hybridization patterns in all investigat ed cytoplasms. For the fertile cytoplasm, as well as ANN4 ANN5, ANL1, ANL2 ARG2 and MAX1, hybridizations obtained with orfH708 were highly polymorphic . Hybridization signals with orfH522 were only detectable in the PET1-like CMS sources and MAX1. Comparing the mitochondrially encoded proteins of the CMS sources characteristic patterns could be detected for seven cytoplasms in addition to the PET1-like CMS sources expressing the 16-kDa protein. Fo r ANN1 and ANN3 three CMS-associated proteins of 16.3 kDa, 16.9 kDa and 34. 0 kDa could be identified among the in organello translation products. Also ANTI expressed three additional proteins of 13.4 kDa, 17.8 kDa and 19.7 kD a, respectively. In ARG3 and RIG1 one protein of 17.5 kDa was missing and i nstead a new protein of 16.9 kDa appeared. In addition, in GIG1 and PET2 a unique protein of 12.4 kDa could be identified. These results indicate that certain types of cytoplasmic male sterility are preferentially present in sunflower.