Estrogen increases Ca2+ efflux from female porcine coronary arterial smooth muscle

Acute estrogen administration relaxes vascular smooth muscle by decreasing intracellular Ca2+ concentration ([Ca2+](i)). In the present study, we exam ined the hypothesis that this reduction in [Ca2+](i) is mediated in part by enhanced Ca2+ efflux. Coronary artery smooth muscle cells were isolated fr om gonad-intact, sexually mature female pigs. The [Ca2+](i) response to end othelin-1 was measured using flue 3 and confocal microscopy. 17-Estradiol ( E(2)beta), but not 17 alpha-estradiol or triamcinolone acetonide, caused a concentration-dependent (IC50 = 10 nM) decrease in the [Ca2+](i) response t o endothelin-1. This decrease was blocked by the specific estrogen receptor antagonist ICI-182780. Under conditions in which Ca2+ influx and sarcoplas mic reticulum Ca2+ reuptake were blocked, E(2)beta still decreased [Ca2+](i ). The response was blocked by extracellular lanthanum. These data indicate that E(2)beta decreases [Ca2+](i) in coronary artery smooth muscle by affe cting Ca2+ efflux via a receptor-mediated mechanism.