Impression Cytology (IC) is a noninvasive and easily repeated technique for
investigating many pathologies of the conjunctiva such as dry eye, drug to
xicities, and melanoma. In addition to skin tests and determination of seru
m IgE levels, the presence of eosinophilia in the nasal mucosa and the conj
unctiva is an important diagnostic factor in allergic rhinoconjunctivitis.
Eosinophilia can classically be shown by the nasal smear technique as well
as IC. The nasal smear is a difficult test to carry out and does not always
give adequate results. With this in mind, we collected cells from the nasa
l mucosa and superior palpebral conjunctiva from 27 patients with allergic
rhinoconjunctivitis (17 female, IO male) using 5 X 5 mm, 0.22 mu m pore-siz
ed cellulose acetate paper. The specimens were fixed in 95% alcohol and wer
e then stained with hematoxylin eosin and periodic acid-Schiff stain for ex
amination by light microscopy. Taking the free and intraepithelial eosinoph
ilia into consideration, the specimens were grouped Of all patients, 92.6%
and 85.2% had nasal and conjunctival eosinophilia, whereas 44.4% and 25.9%
had nasal and conjunctival mononuclear cells, respectively. Our study has s
hown that IC is a very reliable and practical technique that offers a valua
ble alternative test for investigating both the intraepithelial and free eo
sinophilia in allergic rhinoconjunctivitis and other pathologies that cause
cytologic changes.