Comparison of a parasite lactate dehydrogenase-based immunochromatographicantigen detection assay (Optimal (R)) with microscopy for the detection ofmalaria parasites in human blood samples

Microscopic examination of blood smears remains the gold standard for malar ia diagnosis, but is labor-intensive and requires skilled operators; Rapid dipstick technology provides a potential alternative. A study was conducted in The Gambia to compare the performance of OptiMAL(R), an immunochromatog raphic antigen detection assay for the diagnosis of malaria using parasite lactate dehydrogenase, against standard microscopy in patients with suspect ed malaria. For initial diagnosis of Plasmodium falciparum, irrespective of stage, this assay had a sensitivity of 91.3%, a specificity of 92%, a posi tive predictive value of 87.2%, and a negative predictive value of 94.7%. T he sensitivity of the test decreased markedly at parasitemias < 0.01%. This assay can be used for the diagnosis of malaria in areas where microscopy i s not available and for urgent malaria diagnosis at night and at weekends, when routine laboratories are closed and when relatively inexperienced micr oscopists may be on duty.