Enantiomeric HPLC separations of 4-substituted-pyrrolidin-2-ones using cellulose based chiral stationary phases

Enantiomeric resolution of 4-substituted-pyrrolidin-2-ones were investigate d on cellulose chiral stationary phase. The separations were made using nor mal phase methodology with a mobile phase of n-hexane-alcohol (methanol, et hanol, 1-propanol or 2-propanol) in various proportions. and a silica-based cellulose tris-benzoate (Chiralcel OJ). The mobile phase was optimized to achieve the best resolution. Effects of variation of alcohol concentration, nature of aliphatic alcohols in the mobile phase were studied. The effects of substitution were analysed. Baseline separation was easily obtained in many cases. The maximum resolution factor obtained was near 7.50.