Citation
Sl. Jordan et al., Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions, APPL ENVIR, 65(3), 1999, pp. 1308-1311
Citations number
28
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240
→ ACNP
Volume
65
Issue
3
Year of publication
1999
Pages
1308 - 1311
Database
ISI
SICI code
0099-2240(199903)65:3<1308:AOKOEC>2.0.ZU;2-T
Abstract
The acid tolerance of Escherichia coli O157:H7 strains can be overcome by a
ddition of lactate, ethanol, or a combination of the two agents. Killing ca
n be increased by as much as 4 log units in the first 5 min of incubation a
t pH 3 even for the most acid-tolerant isolates. Exponential-phase, habitua
ted, and stationary-phase cells are all sensitive to incubation with lactat
e and ethanol. Killing correlates with disruption of the capacity for pH ho
meostasis. Habituated and, stationary-phase cells can partially offset the
effects of the lowering of cytoplasmic pH.