Calmodulin functions as an activator of Pur alpha binding to single-stranded purine-rich DNA elements (PUR elements)

Citation
Ch. Kuo et al., Calmodulin functions as an activator of Pur alpha binding to single-stranded purine-rich DNA elements (PUR elements), BIOC BIOP R, 255(2), 1999, pp. 406-411
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
255
Issue
2
Year of publication
1999
Pages
406 - 411
Database
ISI
SICI code
0006-291X(19990216)255:2<406:CFAAAO>2.0.ZU;2-U
Abstract
Pur alpha is a single stranded DNA-binding protein and binds to a consensus sequence (GGN)n. We have reported that the DNA-binding activity of a singl e stranded cyclic AMP response element-binding protein (ssCRE-BP) is suppre ssed in cerebellum treated chronically with morphine, ssCRE-BP is identical to Pur alpha and the DNA binding activity of Pur alpha is markedly enhance d by a heat stable activator in the nuclear extract. In this report, we pur ified this activator. The amino acid composition and partial amino acid seq uence were determined to be identical to those of calmodulin (CaM), which e nhanced the binding of GST-Pur alpha to various PUR elements in the 5' non- coding regions of the neuropeptide Y, myelin basic protein and nicotinic Ac h receptor beta 4 subunit genes. The data suggest a novel gene expression p athway mediated by Ca/CaM-Pur alpha which may regulate a variety of genes i n addition to those regulated through the CREB pathway. (C) 1999 Academic P ress.