Reduction of nitrite by glycosylated amino acids and glycosylated albumin

Glycosylated amino acids and glycosylated human serum albumin reduce nitrit e to nitric oxide under anaerobic conditions. The amount of nitric oxide pr oduced was recorded by generation of nitrosoHb from deoxyHb. Without preinc ubation after the addition of sodium nitrite, glucose or a mixture of gluco se with amino acid or serum albumin did not cause spectrophotometrically de tectible transformation of deoxyHb into nitrosoHb. The generation of NO inc reased with an increase in content of colored "final" products of amino aci d and serum albumin glycosylation in the incubation mixture. The incubation of blood plasma of patients with diabetes mellitus with nitrite also resul ted in the increased production of NO as compared to blood plasma of health y subjects. During the incubation of healthy subjects' blood plasma with ni trite a small amount of NO was produced. The removal of low-molecular-weigh t compounds was accompanied by a significantly decreased generation of NO b y blood plasma.