Citation
Js. Choi et al., Poly(ethylene glycol)-block-poly(L-lysine) dendrimer: Novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA, BIOCONJ CHE, 10(1), 1999, pp. 62-65
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOCONJUGATE CHEMISTRY
ISSN journal
10431802
→ ACNP
Volume
10
Issue
1
Year of publication
1999
Pages
62 - 65
Database
ISI
SICI code
1043-1802(199901/02)10:1<62:PGDNLP>2.0.ZU;2-Q
Abstract
Methoxypoly(ethylene glycol)-block-poly(L-lysine) dendrimer was designed to
form a water-soluble complex with plasmid DNA. The copolymer was synthesiz
ed by the liquid-phase peptide synthesis method. It was characterized by H-
1 NMR and matrix-assisted laser desorption ionization-time-of-flight (MALDI
-TOF) mass spectrum. Agarose gel electrophoresis and DNase I protection ass
ay proved that this linear polymer/dendrimer block copolymer assembled spon
taneously with plasmid DNA, forming a water-soluble complex which increased
the stability of the complexed DNA. Atomic force microscopy of the complex
was evaluated at various charge ratios showing that the copolymer/DNA comp
lex was like a globular shape.