H. Kaminaka et al., Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.), BIOS BIOT B, 63(2), 1999, pp. 302-308
We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The de
duced amino acid sequence consists of a polypeptide with 255 amino acids, i
ncluding a putative transit peptide (40 a.a.) in amino-terminal residues. T
his sequence is similar to the known plant Fe-SODs but not classified in th
e group of known Fe-SODs. The metal analysis and Son assays of the partial
purified recombinant protein expressed in E. coli showed that this cDNA enc
odes an iron-containing SOD. However this SOD activity was not inhibited by
the treatment with hydrogen peroxide, which was expected to inhibit known
Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues
examined, being especially abundant in calli, and strongly increased by li
ght induction. These results suggested that this cDNA encodes rice Fe-SOD,
which is apparently distinct from known plant Fe-SODs.