Peptide maps

Authors
Citation
H. Vankova, Peptide maps, CHEM LISTY, 93(2), 1999, pp. 120-127
Citations number
68
Categorie Soggetti
Chemistry
Journal title
CHEMICKE LISTY
ISSN journal
00092770 → ACNP
Volume
93
Issue
2
Year of publication
1999
Pages
120 - 127
Database
ISI
SICI code
0009-2770(1999)93:2<120:PM>2.0.ZU;2-W
Abstract
Peptide mapping is common techique used for studying proteins. Such methods can explain small changes in the structure of the protein molecule. The ar ticle deals with various types of methods which are used for this purpose. The article describes first hydrolysis of different enzymes or degradation with cyanogen bromide, then separation of peptide fragments (by chromatogra phy and electrophoresis). Finally, the separated peptides are detected. The individual steps of the technique are reviewed.