TE671 cell-based ELISA for anti-acetylcholine receptor antibody determination in myasthenia gravis

Background: Acetylcholine receptor (AChR) from human muscles is the antigen used currently in radioimmunoprecipitation assays (RIPAs) for the determin ation of anti-AChR antibodies in the diagnosis of myasthenia gravis (MG), O ur aim was to develop and validate an ELISA using TE671 cells as the source of AChR, Methods: After TE671 cell homogenization, the crude AChR extract was used f or plate coating. Anti-AChR antibodies were determined in 207 MG patients a nd in 77 controls. Results: The mean intra- and interassay CVs (for two samples with different anti-AChR antibody concentrations) were 9.7% and 15.7%, respectively. Test sensitivity and specificity, for generalized MG, were 79.5% (95% confidenc e interval, 72.8-85.0%) and 96.1% (89.0-99.1%), The detection limit was 2 n mol/L, Anti-AChR antibody concentrations from 53 MG patients, as tested wit h our ELISA, showed good agreement with an RIPA with a mean difference (SD) of 1.0 (5.6) nmol/L. Conclusion: Our ELISA is a simple screening test for the diagnosis of MG an d enables rapid and inexpensive patient follow-up, (C) 1999 American Associ ation for Clinical Chemistry.