This article focuses on the most recent research efforts by the Wang group
in the field of alpha-Gal oligosaccharides. alpha-Gal oligosaccharides are
carbohydrate structures bearing a Gal alpha 1-3Gal beta terminus. This clas
s of compounds are believed to act as xenoactive antigens that instigate th
e hyperacute rejection in xenotransplantation. Enzymatic methods using reco
mbinant alpha 1-3 galactosyltransferase were employed to synthesize several
alpha- Gal oligosaccharides. In addition, a chemical synthetic scheme was
devised in order to produce readily acessible amounts of alpha-Gal. Conform
ational analysis was done using both NMR techniques and molecular modeling
protocols. These studies provide important information in the structure-fun
ction relationship of alpha-Gal and anti-alpha-Gal antibodies.
The second part of this article deals with the use of alpha-Gal in immunoth
erapy. Based on the abundance of anti-alpha-Gal antibodies IgM and IgG in h
uman blood serum, alpha-Gal conjugates may act as effective immunotheraputi
c drugs. Recent studies have discovered characteristic peptide sequences co
ntaining Arg-Gly-Asp (RGD) motif that binds to integrins on the surface of
cancer cells. A alpha-Gal conjugate was developed by chemical coupling a al
pha-Gal trisaccharide to a RGD peptide. Preliminary studies using the conju
gate on prostate DU-145 cancer cells showed a reduction in the survival rat
e of the cells.