The release of intracellular calcium (Ca2+) via either inositol 1,4,5-trisp
hosphate receptors (IP3R) or ryanodine receptors (RyR) activates a wide var
iety of signaling pathways in virtually every type of cell. In the present
study we demonstrate that at early stages of development IP3R mRNA and func
tional IP3 gated Ca2+ release channels are widely expressed in virtually al
l tissues in murine embryos. As organogenesis proceeds, more specialized Ry
R channels are expressed in many cell types and the triggering mechanisms f
or intracellular Ca2+ release become more diverse to include IP3-dependent
and voltage-dependent and Ca2+-induced Ca2+ release. As development proceed
s virtually all cell types continue to express IP3R channels but in excitab
le cells including skeletal and cardiac muscles the major Ca2+ release chan
nels are RyRs. This developmental switch from predominantly IP3-mediated to
both IP3-mediated and IP3-independent pathways for intracellular Ca2+ rele
ase is consistent with data showing that IP3R plays an important regulatory
role in cellular proliferation and apoptosis, whereas RyR is required for
other cellular functions including muscle contraction. (C) 1999 Academic Pr
ess.