De. Salt et al., Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy, ENV SCI TEC, 33(5), 1999, pp. 713-717
Citations number
28
Categorie Soggetti
Environment/Ecology,"Environmental Engineering & Energy
Using the noninvasive technique of X-ray absorption spectroscopy (XAS), we
have been able to determine the ligand environment of Zn in different tissu
es of the Zn-hyperaccumulator Thlaspi caerulescens (J. & C. Presl.). The ma
jority of intracellular Zn in roots of T. caerulescens was found to be coor
dinated with histidine. In the xylem sap. Zn was found to be transported ma
inly as the free hydrated Zn2+ cation with a smaller proportion coordinated
with organic acids. In the shoots, Zn coordination occurred mainly via org
anic acids, with a smaller proportion present as the hydrated cation and co
ordinated with histidine and the cell wall. Our data suggest that histidine
plays an important role in Zn homeostasis in the roots, whereas organic ac
ids are involved in xylem transport and Zn storage in shoots.