Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy

Citation
De. Salt et al., Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy, ENV SCI TEC, 33(5), 1999, pp. 713-717
Citations number
28
Categorie Soggetti
Environment/Ecology,"Environmental Engineering & Energy
Journal title
ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN journal
0013936X → ACNP
Volume
33
Issue
5
Year of publication
1999
Pages
713 - 717
Database
ISI
SICI code
0013-936X(19990301)33:5<713:ZLITMH>2.0.ZU;2-#
Abstract
Using the noninvasive technique of X-ray absorption spectroscopy (XAS), we have been able to determine the ligand environment of Zn in different tissu es of the Zn-hyperaccumulator Thlaspi caerulescens (J. & C. Presl.). The ma jority of intracellular Zn in roots of T. caerulescens was found to be coor dinated with histidine. In the xylem sap. Zn was found to be transported ma inly as the free hydrated Zn2+ cation with a smaller proportion coordinated with organic acids. In the shoots, Zn coordination occurred mainly via org anic acids, with a smaller proportion present as the hydrated cation and co ordinated with histidine and the cell wall. Our data suggest that histidine plays an important role in Zn homeostasis in the roots, whereas organic ac ids are involved in xylem transport and Zn storage in shoots.