[C-14]pyrene bound residue evaluation using MIBK fractionation method for creosote-contaminated soil

The fate of [C-14]pyrene was evaluated in creosote-contaminated soil underg oing remediation in a prepared bed system at the Champion International Sup erfund site in Libby, MT. C-14-bound residue formation was evaluated using the methyl isobutyl ketone (MIBK) humic fractionation procedure, and it inc reased through 294 days of incubation in biologically active microcosms for humic acid, fulvic acid, bound humic acid, and mineral-associated organic carbon fractions. The relative affinity of the added pyrene and transformat ion products was highest for the humic acid fraction. Bound residue formati on in PAH-contaminated soil was observed to be an important fate mechanism in the prepared bed system and may be an acceptable end point in the remedi ation of contaminated soil.