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ENG

Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

Authors

Nakamura, K Fujita, A Murata, T Watanabe, G Mori, C Fujita, J Watanabe, N Ishizaki, T Yoshida, O Narumiya, S

Citation

K. Nakamura et al., Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail, FEBS LETTER, 445(1), 1999, pp. 9-13

Citations number

Categorie Soggetti

Biochemistry & Biophysics

Journal title

FEBS LETTERS

ISSN journal

00145793 → ACNP

Volume

445

Issue

Year of publication

1999

Pages

9 - 13

Database

ISI

SICI code

0014-5793(19990219)445:1<9:RASGRP>2.0.ZU;2-L

Abstract

Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-bi nding protein, were examined in mice. Rhophilin mRNA was highly expressed i n adult testis, but was absent in the testis of WIWV mice deficient in germ cells.,An anti-rhophilin antibody detected a band of an expected size in s perm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail, These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm t ail. (C) 1999 Federation of European Biochemical Societies.