Lipase - Catalyzed glycerolysis of sunflower oil to produce partial glycerides

Partial glycerides were prepared by glycerolysis of sunflower oil in presen ce of lipase enzyme as catalyst. Six lipases of different origins were used and compared for their catalytic activity. These include Chromobacterium l ipase, pancreatic lipase, Rhizopus arrhizus lipase, lyophilized lipase (pla nt lipase) in addition to two lipase preparations derived from Rhizopus jap onicus; Lilipase A-10 and Lilipase B-2. Chromobacterium lipase was found to be the most active as glycerolysis catalyst whereas lyophilized lipase; a plant preparation from wheat germ was the least active. The results have also shown that the lipase type affects also the product p olarity and hence its field of application as a food emulsifier. Less polar products can be obtained using Chromobacterium lipase whereas the more pol ar ones using a fungal lipase preparation Lipase A-10,,. The product polari ty is also influenced by the process temperature but the mode of its effect is different for different lipases.