On-line coupling of separation techniques to NMR

The hyphenation of chromatographic separation techniques with NMR spectrosc opy is one of the most powerful and time-saving methods for the separation and structural elucidation of unknown compounds and molecular compositions of mixtures. Most of the routinely used NMR now-cells have detection volume s between 40-180 mu L for conventional separations with analytical columns, and the newest designs employ detection volumes in the order of 200 nL for capillary separations, The low flow rates used in capillary chromatography permit the use of deuterated solvents. Unequivocal structural assignment o f unknown chromatographic peaks is possible by two-dimensional stopped-flow capillary HPLC-NMR experiments.