A novel PCR-based approach for the detection of the Huntington disease associated trinucleotide repeat expansion

Huntington disease (HD) is an autosomal dominant neurodegenerative disorder associated with expansions of an unstable CAG trinucleotide repeat in exon 1 of the IT15 gene. In normal individuals, IT15 contains up to 35 CAG repe ats, while in affected the repeat length is >36, Polymerase chain reaction (PCR) is used to estimate the number of CAG repeats but may be inefficient in long repeats because of the high C+G content of the HD locus. We present a novel PCR approach for the diagnosis of HD, which permits direct visuali zation of the amplified products on agarose gel, using ethidium bromide. It is based on the methylation sensitive conversion of C residues to U by bis ulfite treatment of single-stranded DNA and subsequent amplification of the sense strand with specific primers. The bisulfite treatment dramatically r educes the C+G content of the region; thus, the high Tm and stable secondar y structures are no longer obstacles to PCR. Zn both normal and affected in dividuals, UAG repeats (5'-CAG-3', before bisulfite treatment) in the sense strand can easily be amplified and visualized on a gel by ethidium bromide staining. The method has considerable advantages compared with other descr ibed PCR-based diagnostic tests for HD. Hum Mutat 13:232-236, 1999. (C) 199 9 Wiley-Liss, Inc.