Our aim was to evaluate the reliability of transrectal ultrasonography in t
he preoperative assessment of congenital vaginal canalization defects. We s
tudied nine patients, six with suspected Rokitansky syndrome and three with
suspected complete transverse septum. Before corrective surgery all the pa
tients underwent pelvic examination, transabdominal and transrectal ultraso
nography. The ultrasonographic findings were compared with the surgical one
s. Transrectal ultrasonography provided an accurate map of the pelvic organ
s showing the precise distances between the urethra and bladder anteriorly,
rectum posteriorly, retrohymenal fovea caudally, and pelvic peritoneum cra
nially. Transrectal ultrasonography produced a picture that corresponded pe
rfectly with the real anatomical situation. Conversely, abdominal ultrasono
graphy provided inadequate images in six of our nine patients, and magnetic
resonance imaging was responsible for a mistaken diagnosis in one patient
with suspected transverse vaginal septum. In conclusion, if our results are
confirmed in larger series, transrectal ultrasonography could be considere
d as a diagnostic procedure of choice in the assessment of vaginal canaliza
tion defects.