Low-molecular weight heparin restores in-vitro trophoblast invasiveness and differentiation in presence of immunoglobulin G fractions obtained from patients with antiphospholipid syndrome

The present study was designed to investigate the effects of immunoglobulin G obtained from patients with antiphospholipid syndrome (APS) on in-vitro models of trophoblast invasiveness and differentiation. We tested the bindi ng of affinity-purified immunoglobulin G to human primary trophoblast cells . These antibodies affected the invasiveness and differentiation of cytotro phoblast cells after binding to the cell surface. In addition, we determine d whether the drugs used to treat APS might be able to restore the trophobl ast functions. Low-molecular weight heparin, in a dose-dependent manner, si gnificantly reduced the immunoglobulin G binding to trophoblast cells and r estored in-vitro placental invasiveness and differentiation. No effect was observed in the presence of acetylsalicylic acid. These observations may he lp in understanding the role of these treatments in women with APS.