The Isabela Crater-Lake is a bright-green, hypersaline lake (68-112.5 mS cm
(-1)) on Isabela Island off the Pacific coast of Nayarit, Mexico. Some sali
ent features were documented in November 1993. It appears meromictic, with
three well-defined strata separated by sharp pycnoclines. Surface water was
warm (32 degrees C) reaching a subsurface (0.5-1 m) maximum temperature (3
3 degrees C), declining gradually to 26.7 degrees C at maximum depth (17.5
m). Dissolved oxygen was near saturation at the surface, attained 145 perce
nt saturation at 0.5 m, but was completely absent by 2.5 m. Eh was maximum
at the surface (123 mV), declining to a minimum at 3 m (-261 mV), and was a
bout -240 mV from 3.5 m to the bottom. The pH varied from 9.3 in surface wa
ters to slightly acid (6.4) in deep anoxic layers. Atypically, NO3 was more
abundant than NH4 in both aerobic and anaerobic strata. PO4 and SiO2 conce
ntrations were extremely high. The planktonic microbial community was forme
d by four groups: bacteria (photosynthetic sulfur bacteria and heterotrophi
c bacteria), phytoflagellates (mainly cryptomonads), heterotrophic nanoflag
ellates (Spumella spp., Bodo spp.) and ciliates (Hypotricha and Oligotricha
). Bacteria occurred throughout the water column, but other biota were rest
ricted to surface waters.