Decreased phospholipase A2 activity in butyrate differentiated HT29 cells

Our earlier work has shown that in butyrate differentiated colonic HT29 cel ls, there is an alteration in phospholipid composition a's compared to cont rol. To know more about these changes, butyrate treated and control cell ho mogenates were incubated,in presence of calcium and phospholipids were anal yzed. It was observed that incubation with calcium was associated with incr ease in lysophosphatidylcholine (lysoPC) and free fatty acids and the incre ase was much higher in control as compared to butyrate treated cells. There was no alteration in lysoPC content. These products are formed by the acti on of phospholipase A2 (PLA2) which is activated by calcium and suggests th at butyrate-induced differentiation is associated with decrease in PLA2 act ivity.