Implementation of a six-dimensional search using the AMoRe translation function for difficult molecular-replacement problems

A six-dimensional molecular-replacement procedure has been implemented usin g Perl scripts and the CCP4 version of the translation function of the mole cular-replacement program AMoRe. These tools have allowed the structure det ermination of CTLA-4 monomer from NMR-derived coordinates, and of a potenti al complex of MurD with a substrate. In both cases other molecular-replacem ent programs, X-PLOR and AMoRe, when used in a conventional manner, either failed or did not yield an obvious solution.