ENHANCEMENT OF RAYLEIGH LIGHT-SCATTERING OF ACID-CHROME-BLUE-K BY PROTEINS AND PROTEIN ASSAY BY THE SCATTERING TECHNIQUE

Rayleigh light scattering of Acid Chrome Blue K (ACBK) is enhanced gre atly by proteins, Based on this, a method for protein assay in aqueous solution was developed, This assay matches the sensitivity of the col orimetric dye-binding method with a linear range of 0.136-10.88 mu g m l(-1). The measurements can be made easily on a common fluorimeter. Th e reaction between ACBK and proteins is completed in 2 min and the sca ttered light signal is stable for at least 3 h, Protein-to-protein var iability is encountered in this method as in many other protein assays , There is little or no interference from amino acids, most metal ions and complexing agents (e.g., EDTA), Interferences from salts, urea an d detergents can be minimized by dilution.