During an acute inflammatory response, endothelial P-selectin (CD62P) can m
ediate the initial capture of neutrophils from the free flowing bloodstream
. P-selectin is stored in secretory granules (Weibel-Palade bodies) amid is
rapidly expressed on the endothelial surface after stimulation with histam
ine or thrombin. Because neutrophil transmigration occurs preferentially at
endothelial borders, we wished to determine whether P-selectin-dependent n
eutrophil capture (adhesion) occurs at endothelial cell borders. Under stat
ic or hydrodynamic now (2 dyn/cm(2)) conditions,, histamine (10(-4) M) or t
hrombin (0.2 U/mL) treatment induced preferential (greater than or equal to
75%) neutrophil adhesion to the cell borders of endothelial monolayers, Bl
ocking antibody studies established that neutrophil adhesion was completely
P-selectin dependent. P-selectin surface expression increased significantl
y after histamine treatment and P-selectin immunostaining was concentrated
along endothelial borders. We conclude that preferential P-selectin express
ion along endothelial herders may be an important mechanism for targeting n
eutrophil migration at endothelial borders.